Phenotype MicroArrays for Mammalian Cells
Supplied By: Biolog, USA
Rs. 0.00
Phenotypic Analysis with Odin
When using Phenotype MicroArrays, you need an instrument that will read at the right wavelengths and measure the changes kinetically over time at short intervals. You also need a software package to help you analyze all that data and identify the differences between your samples. With the Odin™ platform you can characterize phenotypes by monitoring growth curves and cell metabolism kinetics for microbial or mammalian cells for up to 50 plates at the same time. When combined with Phenotype MicroArrays, Odin can analyze up to 4,800 conditions unattended for hours or days.
Phenotype Microarrays™ for Mammalian Cells
Phenotype MicroArray Mammalian assays are cell-based assays used to investigate up to 1,400 metabolic and chemical sensitivity phenotypes.
They use 96-well plates pre-loaded with carbon and nitrogen substrates, ions, stimulatory and inhibitory compounds such as hormones/cytokines and anti-cancer agents. Cells of interest are added to the plates along with Biolog’s proprietary redox dye. The generation of energy-rich NADH by the cells reduces the redox dye and brings about a color change which can be read and analyzed by Odin.
Applications
Phenotype MicroArray Mammalian Assays can be used for research in a diverse range of applications, including:
Correlating phenotypes with genotypes
Cell line and bioprocess improvement
Cellular metabolism, metabolic disorders, nutrition
Cell energetics, growth and death
Cell line QC and authentication
Drug discovery research and toxicity screening
Studying metabolic reprogramming in cancer and anti-cancer drug sensitivity
There are 14 panels designed to interrogate metabolic pathways along with ionic, osmotic and pH effects, and 10 panels to assess the sensitivity to various antimicrobials with different mechanisms of action.
| Plate Name | Description |
| PM-M 1-4 Microplates | Mammalian metabolite utilization assays |
| PM-M 5 Microplate | Mammalian cation/anion sensitivity assays |
| PM-M 6-8 Microplates | 100 to 240 volts, 50 to 60 Hz |
| PM-M 11-14 Microplate | Mammalian chemosensitivity assays |
Checking for Growth with Odin
See what’s happening in two ways.
Odin measures a reporter dye over time at one wavelength to measure NADH production, effectively reporting the rate of metabolic respiration.
It also measures optical density (OD) at a second wavelength to determine how quickly the microbes are dividing. Taken together, you get a full picture of how your microbes grow best over time.
If you’re focused on cell growth, just leave the dye out. Odin automatically compares cell growth and metabolic activity curves to provide you with a clearer picture of how fast or slow your microbes are growing.
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